| Webster's Online Dictionary |
| Expressions | Definition | ||
| Fluorescent in situ hybridization | FISH (Fluorescent in situ hybridization) is a cytogenetic technique which can be used to detect and localize DNA sequences on chromosomes. It uses fluorescent probes which bind only to those parts of the chromosome with which they show a high degree of sequence similarity. Fluorescence microscopy can be used to find out where the fluorescent probe bound to the chromosome. (references) | ||
| In situ hybridization | In situ hybridization is a biochemical method for determining the spatial expression pattern of specific genes. There are several ways to image the location of hybridized gene product including immunohistochemistry, fluorescence, and radioactive labeling. (references) | ||
Source: compiled by the editor from various references; see credits. | Top | ||
| Expressions | Domain | Definition | |
| Fluorescence in situ hybridization | Health | A physical mapping approach that uses fluorescein tags to detect hybridization of probes with metaphase chromosomes and with the less-condensed somatic interphase chromatin. (references) | |
| In situ hybridization | Geology | Use of a DNA or RNA probe to detect the presence of the complementary DNA sequence in cloned bacterial or cultured eukaryotic cells. Also used for locating genes on chromosomes. The process is: 1) Prepare microscope slide with cells in metaphase of mitosis; 2) Treat slide with a weak base. Thus denaturing the DNA; 3) Pour radioactively labeled probe onto the slide; 4) Expose slide to photographic emulsion for a few days or weeks; and 5) Develop emulsion. (references) | |
| In situ hybridization | Health | 1: The base pairing of a sequence of DNA to metaphase chromosomes on a microscope slide. (references) | |
| 2: A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes. (references) | |||
Source: compiled by the editor from various references; see credits. | Top | ||
